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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmiah Perikanan dan Kelautan BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) JFMR (Journal of Fisheries and Marine Research) Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Jurnal Layanan Masyarakat (Journal of Public Service)
Heru Pramono
Department Of Marine, Faculty Of Fisheries And Marine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Social Sciences Other

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Journal : ILMU KELAUTAN: Indonesian Journal of Marine Sciences

 1 
Isolation of Lactic Acid Bacteria That Produce Protease and Bacteriocin-Like Substance From Mud Crab (Scylla sp.) Digestive Tract (Isolasi Bakteri Asam Laktat yang Menghasilkan Protease dan Senyawa Bacteriocin-Like dari Saluran Pencernaan Kepiting Heru Pramono; Pipin Suciati; Sapto Andriyono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.947 KB) | DOI: 10.14710/ik.ijms.20.1.33-37

Abstract

Saluran pencernaan merupakan lingkungan kompleks yang terdiri atas berbagai spesies bakteri. Saluran pencernaan biota perairan terdiri atas bakteri aerob dan anaerob yang mampu memproduksi senyawa antibakteri dan enzim. Tujuan penelitian ini adalah untuk mengisolasi bakteri asam laktat yang menghasilkan protease dan senyawa bakteriosin-like dari saluran pencernaan kepiting bakau. Isolasi dan karakterisasi isolat dilakukan menggunakan media MRS. Supernatan netral bebas sel isolat telah diuji dengan menggunakan disc difusi agar terhadap bakteri patogen dan pembusuk. Uji produksi enzim protease telah diuji dengan metode disc diffusion agar menggunakan media kasein agar. Di antara seratus isolat, terdapat 96 isolat menunjukkan zona bening di MRS + CaCO3,, katalase negatif, dan bakteri Gram positif. Tiga puluh empat isolat bakteri asam laktat menghasilkkan protease dan hanya empat isolat (yaitu IKP29, IKP30, IKP52, dan IKP94) menunjukkan penghambatan yang kuat terhadap bakteri patogen dan pembusuk. Terdapat tiga pola inhibisi dari keempat isolate terhadap Bacillus subtilis, Staphylococcus aureus, Eschericia coli, dan Salmonella sp. Empat isolat tersebut berpotensi untuk dimanfaatkan sebagai starter pada produksi fermentasi produk hasil perikanan. Penelitian ini adalah penelitian pertama terkait isolasi bakteri asam laktat yang menghasilkan protease dan bakteriosin dari saluran pencernaan dari kepiting bakau.Kata kunci: Bakteri Asam Laktat, Bakteriosin-like substance, Protease, Scylla  sp. Digestive tract is complex environment consist of large amount of bacteria’s species. Fish intestine bacteria consist of aerobic or facultative anaerob bacteria which can produce antibacterial and enzym. The objectives of this research were to isolated lactic acid bacteria that produce bacteriocin-like and protease from mud crab digestive tract. Isolation and characterization of isolates were conducted employing media MRS.  Neutralized cell free supernatant of isolates were tested using disc diffusion agar of against pathogenic and spoilage bacteria to indicate bacteriocin-like-producing lactic acid bacteria. Protease-producing isolate was tested using disc diffusion method in casein agar. Among a hundred isolates, 96 isolates were showed clear zone in MRS+CaCO3,, catalase negative, and Gram positive bacteria. Thirty four isolates produced protease and only four isolates (i.e. IKP29, IKP30, IKP52, and IKP94) showed strong inhibition against pathogenic and spoilage bacteria. There were three patterns of inhibition among three isolates against Bacillus subtilis, Staphylococcus aureus, Eschericia coli, and Salmonella sp. All three isolates showed potential uses for produce starter culture for fishery product fermentation purpose. This is the first report of isolation lactic acid bacteria that produced protease and bacteriocin-like from digestive tract of mud crab. Keywords: Lactic acid bacteria, Bacteriocin-like substance, Protease, Scylla  sp.
First Record of The Burrowing Goby Trypuchen vagina from Pangpang Bay, Indonesia Sapto Andriyono; Endang Dewi Masithah; Heru Pramono; Suciyono Suciyono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 3 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.522 KB) | DOI: 10.14710/ik.ijms.24.3.127-131

Abstract

The burrowing goby, Trypauchen vagina, is recorded for the first time from the Pangpang Bay of Banyuwangi, Indonesia. Types of fish T. vagina is a type that has not published in Indonesia. This fish was caught using trap net in waters Pangpang Bay, which is an essential area in Banyuwangi, East Java. The description of the morphology and fish habitat conditions shows that the region has a diversity of fish resources that need attention. This goby fish has a local name as fish bedhul picek due to the sense of sight on these fish as if it does not work. Eyes covered by skin and socket head section was not found their eyeballs. The body of the specimens is elongate and reddish-pink in fresh specimens in coloration. There is no barbel on the chin. Cycloid scales are present on the whole body except for the head. Muncar people commonly consume T.vagina. This fish has been food sources in several regions but uncommon at several local markets in Indonesia. The T.vagina inhabit at Pangpang Bay as the bay, which has the high primary productivity of these waters due to sufficient nutrient support. Enrichment of nutrient comes from the run-off land and also the input of the number of fishing activity (embankment). An earlier report said that this fish found in waters Banyu Asin, Palembang, Sumatra, Indonesia. It also confirms that the fish found in this study are also living in a mud substrate that is reasonably smooth with right mangrove conditions around the bay, which close to Alas Purwo National Park.
Co-Authors Abdillah, Annur Ahadi Abdul Azis Agustono - Ahmad Zainal Fanani Alief Ayu Selia Dewi, Siti Nur Rukhoiyah Dwi Yuli Pujiastuti Eka Saputra Emy Koestanti Sabdoningrum Endang Dewi Masithah Fanani, Ahmad Zainal Fareza Ferdyna Nanda Sumartono Faris Abidin Hafizh M. Noor Nova Andika Novitasari, Tisa Ayu Nur Aida Harahap Pipin Suciati Pipin Suciati Pipin Suciati Pulung Siswantara Pursetyo, Kustiawan tri Rofi’ah, Siti Choiriah Sapto Andriyono Siti Choiriah Rofi’ah Siti Eliana Siti Nur Rukhoiyah Dewi Siti Sahatul Fatimah Sofi Maulana Sri Utari Suciyono, Suciyono Taruna Fernando Putra Tisa Ayu Novitasari Tjahjaningsih, Wahju Tri Wahyuni Wahju Thajaningsih Wahyu Ardianto Warsito, Sunaryo Hadi